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INRA CEPIA/MICA

Exploring enzyme diversity within Polyporales for biomass deconstruction (Polyporales) 2012-2013

The order of Polyporales (Basidiomycetes) is particularly rich in fungi with high biotechnological potential and shows a large diversity of enzymatic lignocellulolytic activities. Exploration of functional diversity within Polyporales is done in collaboration with the Joint Genome Institute in a program aimed at sequencing the genome of 40 fungal strains. Within the framework of the INRA Project Polyporales, the enzymatic machineries of five strains are compared at the genome, transcriptome and proteome level after fungus growth on cellulosic, hemicellulosic or complex lignocellulosic substrates (wheat straw, pine, aspen). The platform molecules (such as oxidized mono- and oligo-saccharides or phenolic compounds) released from the biomass are analyzed by medium-throughpout analytical methods.

Collaborations :

-        DOE Joint Genome Institute, California, US (http://www.jgi.doe.gov/)

-        CAZy, Glycogenomics group, AFMB, Marseille, France (http://www.cazy.org/)

-        Plateforme d'Analyse Protéomique de Paris Sud Ouest (PAPPSO), GIS IBISA, Jouy en Josas, France (http://pappso.inra.fr/index.php?lang=en)

-        CIRM-CF, Collection International de Ressources Microbiennes-Champignons Filamenteux, Marseille, France (https://www6.inra.fr/cirm)

Contact : marie-noelle.rosso@univ-amu.fr

 

INRA CEPIA/MICA Incitative Action 

Microbial Enzymes active on the recalcitrant fraction of Lignocellulose (zyMiLi) 2011-2012

The proposed approach in this exploratory project is to identify the enzymatic activities that deconstruct recalcitrant fractions of lignocellulose specifically targeting the hemicellulose-lignin bonds. The experimental approach will not only identify components that can overcome the limitations already proven commercial enzyme cocktails, but it will also perfectly capable of identifying uncharacterized biochemically proteins so far.

 Collaborations:

- UR 1268 - Biopolymères - Intéractions – Assemblages (BIA) Nantes

- UMR 792 - Laboratoire d’Ingénierie des Systèmes Biologiques et des Procédés (LISBP) Toulouse

 Contact : Jean-Guy.berrin@univ-amu.fr

 

INRA CEPIA Incitative Action

Behavior of enzymes breakdown in models of cell wall assembly (ENZYDAM) 2011-2012

The main objective of the project is to understand the limitations to enzymatic degradation of cell wall polysaccharides and lignin, in networks of polymers that mimic some arrangements present in plant cell walls. In particular, the mobility properties of proteins and their degree of interaction with the building blocks will be comparatively examined in two types of cellulose assembly mimicking different levels of organization: cellulose / xyloglucan / pectin or cellulose / hemicellulose / lignin. This program aims to evaluate the influence of the chemical complexity and densification systems on the mode of action of enzymes and mobility through the use of model systems.

Collaborations:

- UR 1268 - Biopolymères - Intéractions – Assemblages (BIA) Nantes

- UMR 614 –  Fractionnement des AgroRessources et Environnement (FARE) Nantes

Contact : eric.record@univ-amu.fr

 

INRA CEPIA (inter-department)Incitative Action  

Sequencing of Pycnoporus sanguineus for discovering new lignocellulose degradation enzymes (Pycnoporus) 2011-2012

The project objective is to achieve structural and functional annotation (by phylogenetic approach) of the Pycnoporus sanguineus genome, annotation of FOLymes (lignin degradation) and CAZymes (cellulose and hemicellulose degradation). In addition, it is planned to compare the genes organization and chromosomal rearrangement between P. sanguineus and Pycnoporus cinnabarinus (tropical vs. temperate). Finally, some genes will be cloned and the corresponding enzymes will be produced and tested for plant biomass biotransformation.

 Collaborations :

- UR0875, Biométrie et Intelligence Artificielle (BIA), Toulouse, Département MIA (Mathématique et Informatique Appliquées)

Contact : eric.record@univ-amu.fr