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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

The first gene with complete-spectrum resistance to root-knot nematodes (RKN) Meloidogyne spp. has been cloned in Prunus.

The first gene with complete-spectrum resistance to root-knot nematodes (RKN) Meloidogyne spp. has been cloned in Prunus. (IPN Team)

Stone fruit crops (Prunus spp.) are severely attacked by the highly polyphagous root-knot nematodes RKN Meloidogyne spp. (notably M. arenaria, M. incognita and M. javanica) and several natural resistance (R) genes, as an alternative to nematicide ban, have been identified and mapped. Because the longevity of these perennial species prolongs the plant-nematode interaction and greatly increases the risk of resistance breakdown, R genes are being pyramided into new Prunus rootstocks. Among them, the Ma gene, triggering a hypersensitive-like reaction with a complete absence of galls, confers a high-level and complete-spectrum resistance and is thus a key component for sustainable control of all RKN. This gene, a member of the TIR-NBS-LRR (TNL) class of genes, has been cloned by a positional approach and validated using Agrobacterium rhizogenes transformation. Its structure is characterized by a huge C-terminal region with five duplicated post-LRR (PL) domains that makes it the longest R gene cloned to date.Mais the second RKN gene cloned (after the tomato Mi reference gene) and the first one with a complete spectrum.

Root-knot nematode (Meloidogyne spp.) symptoms on susceptible plant (left) and resistant plant with the Ma gene (right)

Root-knot nematode (Meloidogyne spp.) symptoms on susceptible plant (left) and resistant plant with the Ma gene (right)

  • Claverie M., Dirlewanger E., Bosselut N., Van Ghelder C., Voisin R., Kleinhentz M., Lafargue B., Abad P., Rosso M.N., Chalhoub B., Esmenjaud D. (2011) The Ma gene for complete-spectrum resistance to Meloidogyne spp. in Prunus is a TNL with a huge repeated C-terminal post-LRR region.Plant Physiology156:779-792.
  • Khallouk, S., Voisin, R., Van Ghelder, C., Engler, G., Amiri, S., Esmenjaud, D. (2011). Histological mechanism of the resistance conferred by the Ma gene against Meloidogyne incognita in Prunus.Phytopathology101: 945-951.
  • Bosselut N., Van Ghelder C., Claverie M., Voisin R., Onesto J.P., Rosso M.N., Esmenjaud D. (2011) Agrobacterium rhizogenes-mediated transformation of Prunus as an alternative for gene functional analysis in hairy-roots and composite plants.Plant Cell Reports30:1313-1326.