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Dernière mise à jour : Mai 2018

Menu Institut Sophia Agrobiotech Inra Univ. Nice Sophia Antipolis CNRS

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Institut Sophia Agrobiotech

UMR INRA - Univ. Nice Sophia Antipolis - Cnrs

http://www.paca.inra.fr/institut-sophia-agrobiotech_eng/

The plant cytoskeleton and cell cycle progression are maneuvered by sedentary phytoparasitc nematodes

The plant cytoskeleton and cell cycle progression are maneuvered by sedentary phytoparasitc nematodes (IPN Team)

Sedentary phytoparasitic nematodes are capable to maneuver the cell cycle in addition to disarray the cytoskeleton in their favor. Our projects focus on the investigation of cell cycle progression and the cytoskeleton organization and dynamics in feeding sites induced by parasitic nematodes. To study the cytoskeletal changes and cell cycle in root cells undergoing feeding cell development we make use of immunocytochemical approaches, in vivo GFP strategies, histological analysis and infection tests to investigate the undergoing changes in both processes within feeding sites at different stages of nematode infection. Functional analysis of lines down-regulated and knockout, or overexpression of genes involved in the cytoskeleton and the cell cycle have been carried out as well. Recently we deciphered the function of g-tubulins as nucleating new microtubule cytoskeleton in organizing centers in feeding sites. We have also identified genes such as CCS52 gene family, DEL1 and RHL1 involved in the endocycle control of gall cell. Moreover, we newly demonstrated that cell cycle inhibitor genes of the ICK/KRP family negatively control gall size and nematode reproduction. A potential link between cell cycle and the cytoskeleton is also an approach that will be pursued.

 

Whole-mount analysis of nuclei in galls induced by Meloidogyne incognita in Arabidopsis roots stained with propidium iodide. Thirty-day-old galls containing a large number of nuclei in giant cells. Asterisk, giant cell; n, nematode. Bars = 20 µm.

Whole-mount analysis of nuclei in galls induced by Meloidogyne incognita in Arabidopsis roots stained with propidium iodide. Thirty-day-old galls containing a large number of nuclei in giant cells. Asterisk, giant cell; n, nematode. Bars = 20 µm.

  • de Almeida Engler, J., Kyndt, T., Vieira, P., Van Cappelle, E., Bouldolf, V., Sanchez, V., Escobar, C., De Veylder, L., Engler, G., Abad, P., Gheysen, G. (2012) CCS52 and DEL1 genes are key components of the endocycle in nematode induced feeding sites.The Plant Journal, 72: 185-198.
  • Vieira, P., Engler, G. & de Almeida Engler, J. (2012). Whole-mount confocal imaging of nuclei in giant feeding-cells induced by root-knot nematodes in Arabidopsis. New Phytologist  195: 488-496.

Related

  • Banora, M.Y.; Rodiuc, N., Baldacci-Cresp, F.; Smertenko, A., Bleve-Zacheo, T., Melillo, M.T., Karimi, M., Hilson, P., Evrard, J.L., Favery, B., Engler, G., Abad, P., de Almeida-Engler, J. (2011) γ-Tubulin Ring Complex is Essential for The Microtubule Reorganisation During Nematode Infection.Plos Pathogens7(12): e1002343.